The Challenges & Solutions of Cryo-FIB Lift-out

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Cryo-focused ion beam (FIB) micromachining of frozen-hydrated cells has provided new and exciting ways to study their inner workings at the ultrastructural and molecular scale. When combined with cryo-electron tomography (cryo-ET), this method allows to explore large macromolecular structures within their native and functional cellular environment. Until recently, this technique has mostly been applied to studies on individual cells that are effectively vitrified by plunge-freezing and routinely thinned by cryo-FIB, leaving more complex multicellular and tissue biology out of reach. The application of cryo-lift-out now enables the production of vitrified lamellae from high-pressure frozen samples, encompassing 3D cell cultures, whole small model organisms and tissues. The key step in this advanced preparation entails a micromanipulator system cooled to deep cryogenic temperature that is used to extract targeted portions from vitrified voluminous specimens and make them amenable to subsequent cryo-ET imaging.

We've partnered with the Royal Microscopical Society (RMS) for this webinar, and will share insights we've learned from users about their challenges, solutions and experiences related to cryo lift-out workflows in context of cryo-correlative light and electron microscopy. This includes discussion about technical solutions, using the OmniProbe 350 cryo, and challenges relating to the handling and transfer of lift-out samples from the FIB-SEM.

Join us and learn:

    • How sample preparation techniques such as site specific lift-out are used in combination with cryo-FIB
    • Status of current solutions offered for cryo lift-out
    • Practical issues to consider in relation to cryo lift-out
    On Demand

    On Demand


    1 hour





    In partnership with RMS


    Kim Larsen - Oxford Instruments
    Senior Product Scientist

    Kim Larsen joined HKL Technology A/S in January 2004. The company was acquired by Oxford Instruments in the following year. Thr...

    Dr Evgenia Zagoriy - European Molecular Biology Laboratory
    Laboratory Officer in Charge

    Evgenia Zagoriy is an expert in cryo-focused ion beam (FIB) preparations of vitrified biological specimens, in situ cryo-electr...

    Dr Edoardo D'Imprima - Humanitas Research Hospital
    Coordinator of CLEM facility

    Edoardo D'Imprima coordinates the upcoming Correlative Light and Electron Microscopy Core at the Humanitas Research Hospital, R...

    Dr Chris Parmenter - nmRC
    CAT suite Manager

    Dr Chris Parmenter holds a MChem and PhD in chemistry and is a seasoned electron microscopist and imaging scientist. His experi...

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    Learn all about the universal method of in situ FIB lift-out for Cryogenic samples.