The Challenges & Solutions of Cryo-FIB Lift-out

Kim Larsen joined HKL Technology A/S in January 2004. The company was acquired by Oxford Instruments in the following year. Thr...Read more >

Kim Larsen joined HKL Technology A/S in January 2004. The company was acquired by Oxford Instruments in the following year. Through his history at HKL Technology A/S and then Oxford Instruments, Kim has worked in several areas including Customer Support, Development and Marketing; always with a strong focus on EBSD. He is currently working as a Senior Product Scientist within the Product Science group.

Evgenia Zagoriy is an expert in cryo-focused ion beam (FIB) preparations of vitrified biological specimens, in situ cryo-electr...Read more >

Evgenia Zagoriy is an expert in cryo-focused ion beam (FIB) preparations of vitrified biological specimens, in situ cryo-electron tomography and cryo-correlative light and electron microscopy (CLEM). She has been working in the group of Julia Mahamid in the Structural and Computational Biology Unit at the European Molecular Biology Laboratory Heidelberg (Germany) since 2017. During her postdoctoral tenure at the Max Planck Institute of Biochemistry and her PhD at the Max Planck Institute of Cell Biology and Genetics, she investigated chromosome biology and the regulation of cell division using state-of-the-art fluorescence light microscopy and biochemical methods. Prior to her doctoral studies, she obtained a Master's degree in Biochemistry at the Faculty of Chemistry of the Aristotle University of Thessaloniki (Greece).

Edoardo D'Imprima coordinates the upcoming Correlative Light and Electron Microscopy Core at the Humanitas Research Hospital, R...Read more >

Edoardo D'Imprima coordinates the upcoming Correlative Light and Electron Microscopy Core at the Humanitas Research Hospital, Rozzano (Italy). During his postdoc at EMBL, he investigated cancer onset in tissues using organoid 3D-cell culture systems. For that, together with industrial partners, he developed a multimodal imaging pipeline to link live cell imaging, CLEM, cryo-FIB/SEM volume imaging, lift-out and cryoET. He completed his PhD at Max Planck Institute of Biophysics, focusing on the structural biology of isolated membrane proteins by single particle cryoEM. Before graduating, Edoardo worked as a staff scientist for Merck Serono Italia.

Dr Chris Parmenter holds a MChem and PhD in chemistry and is a seasoned electron microscopist and imaging scientist. His experi...Read more >

Dr Chris Parmenter holds a MChem and PhD in chemistry and is a seasoned electron microscopist and imaging scientist. His experience ranges from cryo-Transmission Electron Microscopy (cryo-TEM) of soft matter systems (polymers, proteins, liposomes, extra cellular vesicles) to focused ion beam scanning electron microscopy (FIB-SEM), and particularly the development of cryo-FIB-SEM sample preparation approaches applied to soft matter systems. He has been developing the cryogenic lift-out technique in conjunction with Oxford Instruments since 2013 to enable cryo-TEM of bulk samples (polymers, cells, tissues). He is based at the University of Nottingham where he is the manager of the Cryogenic Analytical and Transfer (CAT) Suite, a category 2 approved microscopy suite with an emphasis on cryogenic and correlative (light and electron) microscopy.

Chris is the Editor-in-Chief of Microscopy and Analysis, a global microscopy publication aimed at the professional microscopy community. Since 2010 has served on the Royal Microscopical Society (RMS) Materials section (2010-2014) and Electron Microscopy committee (2012-present). He is a board member of Journal of Microscopy and since 2022 is the Chair of the Cryo Microscopy Group.