OI Academy | Application Webinar
Inside Out: Unlocking the Hidden Apical Surface of Organoids for High-Resolution Imaging — Rerun
Timezone: 14:00 TST/SGT | 15:00 KST/JST | 17:00 AEDT
With real-time translation and captioning provided in English, Traditional Chinese, Japanese and Korean (APAC-friendly time).
Traditional organoids hide their functional apical surface within an inaccessible lumen, creating an imaging “black box.”
In this webinar, discover how the phospholipids LPA and S1P, found in serum and CSF, can flip organoids inside-out within 24 hours through GPCR/RhoA signaling. This exposes the apical surface for high-resolution whole-mount imaging of tight junctions, cilia, and subcellular structures—without cryosectioning.
The stable, ECM-free platform can be applied across brain, lung, and intestinal organoids, with reversed polarity maintained for over one month. This enables extended longitudinal studies of barrier function and morphology, while reducing the need for expensive hydrogels and labor-intensive processing.
You will learn about:
- LPA and S1P as universal polarity switches: How LPA and S1P reverse organoid orientation within 24 hours via GPCR/RhoA signaling, externalizing the apical surface across brain, lung, and intestinal tissues.
- High-resolution subcellular imaging: How apical-out organoids enable 60× whole-mount confocal imaging of tight junctions, primary cilia, and microtubules without cryosectioning.
- Long-term experimental stability: How reversed polarity persists for over one month, supporting longitudinal studies of barrier function and morphology.
- Streamlined, cost-effective workflow: How this ECM-free approach eliminates the need for expensive hydrogels and labor-intensive processing, providing a versatile platform for disease modeling.
A live Q&A session will follow the presentation, hosted by Angela Oh, Application Scientist at Oxford Instruments.
On Demand
On Demand
Duration:60 minutes
Language:English
Businesses:Imaris, OI Academy
