Seeing through the Murine Heart - Quantitative 3D Assessment of Myocardial Ischemia/Reperfusion Injury and Response

During this webinar, speakers present a three-dimensional (3D), simultaneous quantitative investigation of key ischemia/reperfusion injury-components by combining bleaching-augmented solvent-based non-toxic clearing (BALANCE) using ethyl cinnamate (ECi) with light-sheet fluorescence microscopy.

Goals:

• Provide insights into anatomical structure of the murine heart in 3D
• Explain the immune response following ischemia/reperfusion injury on an organ level
• Show examples of quantitative light-sheet fluorescence microscopy (LSFM) in highly autofluorescent organs
• Provide examples for semi-manual tracing, spot-function and filament model in Imaris to quantify 3D LSFM data

Featuring the research recently published in Nature Communications: https://www.nature.com/articles/s41467-019-10338-2