Dendritic Spine Segmentation

In this tutorial, learn three methods for dendritic spine segmentation in Imaris 11.0: manual drawing and editing, automatic detection in the Filament wizard, and automatic detection using the Calculate Diameter with Threshold option. This short tutorial demonstrates how to reconstruct and trace dendritic spines in 3D from microscopy images to enable the quantification of morphological measurements such as length, diameter, and volume.